Ricin toxin-resistant clone derived from HEK293S by ethylmethanesulfonate (EMS). Used for fast and easy generation of a stably transfected cell pool by co-transfecting a Flp-InTM expression vector containing a gene of interest and a Flp recombinase expression vector. Has an enhanced ability to produce recombinant protein compared to HEK293-EBNAĭerived from 293 cells by stable transfection of an FRT-site containing plasmid and of a TetR expression plasmid. This cell line is transfected with EBNA1t, a truncated version of EBV EBNA1 lacking the Gly-Gly-Ala repeats region Later adapted to growth in serum-free medium (SFM) (GIBCO 293H)įast growth in SFM, good adherence during plaque assays, superior transfection efficiencies and a high level of protein expressionĮxpresses the EBNA-1 protein for episomal replication of oriP harboring plasmidsĪmplification of vectors containing oriP, considerably increasing the protein expression levels during transient transfection. Growth in chemically defined mediumĬloned from HEK293 to select a clone with good adherence during plaque assays. Stable transfection of the HEK 293 cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigenĪmplification of vectors containing the SV40 ori, considerably increasing the protein expression levels during transient transfection.Įxpresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.Ĭloned from the HEK293 cell line and adapted to commercial mediumįast growth and high transfectivity. Grows in suspension in modified minimal Eagle’s medium Transformation of Human embryonic kidney cells with sheared fragments of adenovirus type 5 (Ad5) DNA, selected for immortalization. This review provides a comprehensive summary of important achievements in HEK293 cell line engineering and on the global engineering approaches and functional genomic tools that have been employed to identify relevant genes for targeted engineering.

However, the modification of genes involved in cellular processes, such as cell proliferation, apoptosis, metabolism, glycosylation, secretion, and protein folding, in addition to bioprocess, media, and vector optimization, have greatly improved the performance of this cell line. At the present time, the growth and production properties of the HEK293 cell line are inferior to those of non-human cell lines, such as the Chinese hamster ovary (CHO) and the murine myeloma NSO cell lines. In addition to its ease of growth in serum-free suspension culture and its amenability to transfection, this cell line’s most important attribute is its human origin, which makes it suitable to produce biologics intended for human use.

The HEK293 cell line has earned its place as a producer of biotherapeutics.

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